Equipment
 

Imaging Core Facility PICMI

Cedric GAGGIOLI, Research Director, DR2 Inserm
 

 

PICMI Equipment

 

« LSM » Upright Confocal Microscope LSM ZEISS

4 solid states lasers (488nm, 633nm, 543nm et 405nm), HAL100 illumination lamp, polarized, analyzers et Wollaston prism for DIC, objectives 10x/0,3/EC Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,95/PlanAPOCHROMAT: 63x/1,40/oil/DIC/Plan-APOCHROMAT, one PMT detector, 1 PC station for images acquisition and analysis, ZEN software

  

« LSM880 » inverted Confocal Microscope LSM 880 ZEISS

laser Argon ion (458nm, 488nm, 514nm), laser HeNe (633nm), laser HeNe (543nm), solide state laser 405nm, HAL100 lmap, analyzers et prism Wollaston for DIC, Motorized x, y et z with PIEZZO, objectives 10x/0,3/EC Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,95/Plan-APOCHROMAT : 63x/1,40/oil/DIC/Plan-APOCHROMAT, two PMT detectors, spectral detector 32 GaAsp, Fast AiryScan, CO2and temperature controller, one PC station for acquisition et one PC station for image analysis, ZEN software

 

« LSFM » Light Sheet Fluorescent MicroscopyZ.1 Zeiss

4 solid states lasers (405nm, 488nm, 543nm et 633nm), two sCMOS PCO.edge cameras, illumination objectives 5X and 10X; Acquisition objectives 5X, 20X et 20X for transparisation. Incubation chamber 5x,20x n=1.33, 5x n=1.45 and 20x n=1.45 for transparisation. CO2and temperature controller, one PC station for acquisition et one PC station for image analysis, ZEN software

 

« DV » Time Lapse - DeltaVisionOlympus

4 diodes 405nm, 488nm, 543nm, 632nm, HAL100 lamp, polarized, analyzer and prism Wollaston for DIC, motorized x, y et z, objectives OLYMPUS 20x/0,75, 60x/1,42/oil, 100x/1,40/oil, CCD sCMOS monochromatic camera, 2048x2048 pixels, 6,45μm x 6,45μm, 15 Bit, CO2and temperature controller, one PC station for acquisition with deconvulation

 

« CytoGen » –Axio Imager Z2 Microscope ZEISS

lampe X-Cite series 120Q, motorized x, y et z, with 8 slides older, objectives 10x/0,45/Plan-APOCHROMAT, 20x/0,5/ EC PlanNEOFLUAR, 40x/0,95/Plan-APOCHROMAT, 63x/1,40/oil/DIC/PlanAPOCHROMAT, 100x/1,40/oil/DIC/Plan-APOCHROMAT, HAD CCD CoolCube monochromatic camera 1360x1024 pixels, 6,45μm x 6,45μm, 12 Bit, 1 PC station for images acquisition and analysis, MetaSystems software

 

« MetaMorph » Time Lapse - Axiovert 200M Microscope ZEISS

lamp HXP 120C, HAL100 lamp, polarized, analyzers and prism Wollaston for DIC, motorized x, y et z, objectives 5x/0,12/ A Plan, 10x/0,25/A Plan, 20x/0,4/LD Plan NEOFLUAR, 32x/0,4/ LD A Plan, 40x/1,30/oil/FLUAR, 63x/1,40/oil/DIC/Plan APOCHROMAT, EMCCD monochromatic camera Photometrics CASCADE II, 1024x1024 pixels, 13μm x 13μm, 16 Bit, CO2and temperature controller, one PC station for acquisition and for image analysis MetaMorph software

 

« HD » Time Lapse - Axio Observer Z1 Microscope ZEISS

Lamp HXP 120C, Colibri (leds) illumination (excitation at 365nm, 470nm, 555nm, 625nm), HAL100 lamp, motorized x, y et z, Definite Focus, objectives 2,5x/0,75/PlanNEOFLUAR, 10x/0,3/Plan-NEOFLUAR, 20x/0,4/Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,6/Plan-NEOFLUAR, 40x/0,75/ EC PlanNEOFLUAR , Optovar (numerical zoom), AxioCam Icc1 ZEISS colored camera, 1388x1038 pixels, 4,65μm x 4,65μm, 8Bit, sCMOS ORCA-Flash 4.0 LT Hamamatsu camerac C11440 2048x2048 pixels, 6,5 μm x 6,5 μm, 16bit, CO2and temperature controller, one PC station for acquisition and for image analysis ZEN2 software.

  

Five PC controllers for image analysis:

  • One station for image analysis LSM (ZEN soft)
  • One station for image analysis LSFM
  • One station for image analysis LSM 880
  • One station for image analysis and deconvolution DeltaVision
  • One station for image analysis with Imaris software
  • One station for image analysis CytoGen
  • One station for image analysis with MetaMorph

 

Facilities & Services